Device

Part:BBa_K2088014

Designed by: Shi Kejian   Group: iGEM16_NAU-CHINA   (2016-10-09)


J23101+RiboJ+M6+GFP+dT

The device contains a promoter(J23101), a riboJ (BBa_K1679038), a regulating site(M6) ，a repoter gene(GFP) and a terminator(B0015). The regulating section M6 can bind the PPDA which could release the RBS to start translating, and the riboJ will stabilize the function of other parts. The GFP can be used as a repoter. In general J23101+riboJ+M6+GFP+B0015.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 881


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Categories
Parameters
None